GVPC Agar for Legionella

Principles:

Yeast extract constitutes a primary nutrient leading to Legionella growth.

Activated charcoal decomposes hydrogen peroxide (toxic metabolic by-product), captures the carbon dioxide and modifies the surface tension.

The ACES/KOH buffer maintains the pH and permits aerobic incubation.

Cysteine and ferric pyrophosphate represent indispensable nutritive elements for the growth of Legionella.

α-cetoglutarate is a growth activator for Legionella.

Secondary microflora are inhibited by the association of glycine, vancomycin, polymyxin B and cycloheximide.