Yeast extract constitutes a primary nutrient leading to Legionella growth.
Activated charcoal decomposes hydrogen peroxide (toxic metabolic by-product), captures the carbon dioxide and modifies the surface tension.
The ACES/KOH buffer maintains the pH and permits aerobic incubation.
Cysteine and ferric pyrophosphate represent indispensable nutritive elements for the growth of Legionella.
α-cetoglutarate is a growth activator for Legionella.
Secondary microflora are inhibited by the association of glycine, vancomycin, polymyxin B and cycloheximide.