3M™ Fish Protein ELISA Kit Quantitative ELISA food allergen detection 3M
3M

3M™ Fish Protein ELISA Kit

7100151353
CAD$643.43

The 3M™ Fish Protein ELISA Kit is intended for the detection of fish proteins in clean-in-place water (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.

(96 tests per kit)

Limit of quantification: 1.0 ppm

QUANTITY

The 3M™ Fish Protein ELISA Kit utilizes a sandwich ELISA. The fish proteins present in the sample react with the anti-fish antibody, which have been adsorbed to the surface of polystyrene microtiter wells.

After the removal of unbound proteins by washing, anti-fish antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound fish protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB).

The colour development from this enzymatic reaction varies directly with the concentration of fish protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of fish protein in the test sample.

The quantity of fish protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.

7100151353
3M
New product
© Innovation Diagnostics© All rights reserved | Sitemap
Loading...