Mustard Protein ELISA Kit
The 3M™ Mustard Protein ELISA Kit is intended for the detection of mustard proteins in clean-in-place water (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.
(96 tests per kit)
Limit of quantification: 1.5 ppm
The 3M™ Mustard Protein ELISA Kit utilizes a sandwich ELISA. The mustard proteins present in the sample react with the anti-mustard antibody, which have been adsorbed to the surface of polystyrene microtiter wells.
After the removal of unbound proteins by washing, anti-mustard antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound mustard protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB).
The colour development from this enzymatic reaction varies directly with the concentration of mustard protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of mustard protein in the test sample.
The quantity of mustard protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.
- Designed to detect both processed and unprocessed target allergen proteins
- Test can accommodate environmental swab, CIP rinse water and food samples
- A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time